Mechanism of Early Flowering in a Landrace Naked Barley eam8.l Mutant

The EARLY MATURITY 8 (EAM8) gene of barley is homologous to the FLOWERING 3 (ELF3) in Arabidopsis, as loss-of-function mutations this circadian clock promote rapid flowering. A previous study demonstrated that early flowering phenotype a hulless barley, Lalu, was due allele eam8.l carrying an alternative splicing mutation intron led retention. In present study, we verified encoded truncated protein. Although EAM8 expressed at higher level Lalu than other lines with longer growth period, it did not negatively regulate time. This result further proved protein nonfunctional regulating barley. plants strongly dependent on biosynthesis gibberellin (GA). stopped suppression GA biosynthesis, and accumulated excessive GA, especially leaves. achieved through upregulated expression genes pathway, including GA20ox2, LFY1, SOC1, PAP2, FPF3. also abolished inhibition LOCUS T-like (FT1) night. During night, levels FT1 were those during day Lalu. However, GA-dependent pathway mechanism are two independent pathways Alleles EAM8, therefore, important breeding value which probably effective many day-length-sensitive crop well; thus, they could be used tune adaptation different geographic regions climatic conditions, critical issue times global warming.